Synopsis "Drosophila Oogenesis: Methods and Protocols"
1. The Vast Utility of Drosophila Oogenesis Michelle S. Giedt and Tina L. Tootle 2. Visualization and Quantification of Drosophila Larval Ovaries Alicia E. Rosales-Nieves, Miriam Marín-Menguiano, Alejandro Campoy-Lopez, and Acaimo González-Reyes 3. Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries Julie A. Merkle 4. Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary Daniel N. Phipps, Amanda M. Powell, and Elizabeth T. Ables 5. Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication Lesley N. Weaver 6. Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes Tingting Duan, Felipe Rodriguez-Tirado, and Pamela K. Geyer 7. Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells Anna E. Williams and Elizabeth T. Ables 8. Tracking Follicle Cell Development Adrianna Soriano, Christopher Petit, Savannah Ryan, and Jennifer C. Jemc 9. Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells Mitchell T. Anderson, Kristin Sherrard, and Sally Horne-Badovinac 10. Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration Yujun Chen, Nirupama Kotian, and Jocelyn A. McDonald 11. Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis Jonathan A. Jackson, Jasmin Imran Alsous, and Adam C. Martin 12. Visualizing Lipid Droplets in Drosophila Oogenesis Roger P. White and Michael A. Welte 13. Assessing Ovulation in Drosophila melanogaster Andrew Beard, Rebecca Oramas, and Jianjun Sun 14. A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments Allison L. Zajac, Audrey M. Williams, and Sally Horne-Badovinac 15. Detection and Assessment of Wolbachia pipientis Infection Lindsay B.M. Nevalainen and Irene L.G. Newton 16. Measuring Transposable Element Activity in Adult Drosophila Ovaries Anikó Szabó, Péter Borkúti, Zoltán Kovács, Ildikó Kristó, Csilla Abonyi, and Péter Vilmos 17. Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing N
