Synopsis "Mechanobiology: Methods and Protocols"
Part I: Quantifying the Mechanical Properties of Cells and Adhesion Proteins 1. Measuring Cell Mechanical Properties Using Microindentation Julien Husson 2. In Situ Measurements of Cell Mechanical Properties Using Force-Spectroscopy Meital Reches 3. Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using Atomic Force Microscopy-Based Force Spectroscopy Martin Bergert and Alba Diz-Muñoz 4. Characterizing the Biophysical Properties of Adhesive Proteins in Live Cells Using Single Molecule Atomic Force Microscopy Andrew Vae Priest and Sanjeevi Sivasankar Part II: Exposing Cells to External Mechanical Forces 5. Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber Jason Sedlak and Alisa Clyne 6. Cell Stretcher Assay to Analyze Mechanoresponses to Cyclic Stretching Robin Püllen, Jens Konrad, Bernd Hoffmann, and Rudolf Merkel 7. Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo Kenji Nishizawa, Claire Chardès, Raphaël Clément and Pierre-François Lenne Part III: Quantitative Characterization of Cell Mechanosensing 8. A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity-Sensing by Plant's Roots and Root Hair Cells David Pereira, Thomas Alline, Gaurav Singh, Marie-Edith Chabouté, and Atef Asnacios 9. Photoresponsive Hydrogels for Studying Mechanotransduction of Cells Keun-Young Park, David J. Odde, and Mark D. Distefano 10. Analyzing Mechanically Evoked Currents at Cell-Substrate Junctions Surabhi Shrestha, Jessica Richardson, and Kate Poole 11. Quantifying Strain Sensing Protein Recruitment During Stress Fiber Repair Shailaja Seetharaman, Stefano Sala, Margaret L. Gardel, and Patrick W. Oakes Part IV: Measuring Forces Applied by Cells on the Extracellular Matrix 12. Quantification of Invadopodia Formation and Matrix Degradation Activity Jubina Balan Venghateri and Benjamin Geiger 13. Measuring Cellular Traction Forces with Micropillar Arrays Malak Amer and Haguy Wolfenson 14. Imaging Cell Adhesive Force at the Single Molecule Level Ying Tu and Xuefeng Wang Part V: Force Measurement Inside Cells 15. Multiplexed Molecular Tension Sensor Measurements Using PIE-FLIM Lukas Win
