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oligonucleotide synthesis: methods and applications
Piet Herdewijn
(Illustrated by)
·
Humana
· Paperback
oligonucleotide synthesis: methods and applications - Herdewijn, Piet
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Origin: U.S.A.
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Synopsis "oligonucleotide synthesis: methods and applications"
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
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The book is written in English.
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