Synopsis "Protein Aggregation: Methods and Protocols"
1. Early aggregation of Amyloid-β (1-42) studied by Fluorescence Correlation Spectroscopy Mercedes Novo, Cibrán Pérez-González, Sonia Freire, and Wajih Al-Soufi 2. Preparation and investigation of crucial oligomers in the early stages of Aβ40 and Aβ42 aggregation Bertrand Morel and Francisco Conejero-Lara 3. Preparation and fractionation of heterogeneous Aβ42 oligomers with different aggregation properties Erica W. Chen and Zhefeng Guo 4. An efficient method of expression and purification of amyloid beta (Aβ1-42) peptide from E.coli Dhiman Ghosh, Marielle Aulikki Wälti, and Roland Riek 5. Solid-state NMR structure of amyloid-β fibrils Beat H. Meier and Anja Böckmann 6. Time-resolved in situ AFM measurement of growth rates of Aβ40 fibrils Peter G. Vekilov and Peter G. Wolynes 7. Monitoring kinetics of pH-dependent aggregation and disaggregation of the Pmel17 repeat domain Dexter N. Dean and Jennifer C. Lee 8.Analysis of Tau: nucleoporin interactions by Surface Plasmon Resonance Spectroscopy Lisa Diez, Larisa E. Kapinos, Roderick Y. H. Lim, and Susanne Wegmann 9. Microfluidic chamber technology to study missorting and spreading of Tau protein in Alzheimer disease Senthilvelrajan Kaniyappan, Varun Balaji, Yipeng Wang, and Eckhard Mandelkow 10. Using FRET-based biosensor cells to study the seeding activity of tau and α-synuclein Katherine N. Maina, Caroline Smet-Nocca, and Gal Bitan 11. Functional applications of stable tau oligomers in cell biology and electrophysiology studies Emily Hill, Kevin G. Moffat, Mark J. Wall, Henrik Zetterberg, Kaj Blennow, and Thomas K. Karikari 12. An additive-free model for tau self-assembly Youssra K Al-Hilaly, Karen E Marshall, Liisa Lutter, Luca Biasetti, Kurtis Mengham, Charles R Harrington, Wei-Feng Xue, Claude M Wischik, and Louise C Serpell 13. Cross-linking mass spectrometry analysis of metastable compact structures in intrinsically disordered proteins Dailu Chen and Lukasz A. Joachimiak 14. A validated method to prepare stable tau oligomers Emily Hill, Kevin G. Moffat, Mark J. Wall, Henrik Zetterberg, Kaj Blennow, and Thomas K. Karikari 15. Light microscopy and dynamic light scattering to study liquid-liquid phase separation of Tau proteins in vitro Janine Hochmair, Christian Exner, Christian Betzel, Eckhard Mandelkow, and Susanne Wegmann 16. Study of tau liquid-liquid phase separation in vitro Solomiia Boyko and Witold K. Surewicz 17. Liquid-Liquid Phase Separation to study the association of proteins in solution Irving Vega and Andrew Umstead 18. Mapping phase diagram of tau-RNA LLPS under live cell coculturing conditions Yanxian Lin, Yann Fichou, Jennifer N. Rauch, Xuemei Zhang, Kenneth S. Kosik, and Songi Han 19. The role of buffers in wild-type HEWL amyloid fibril formation mechanism - a methodological approach Sandi Brudar and Barbara Hribar-Lee 20. Reproducible formation of insulin superstructures: amyloid-like fibrils, spherulites and particulates Camilla Thorlaksen, Martin Busch Neergaard, Minna Groenning, and Vito Foderà 21. CD and solid-state NMR studies of low-order oligomers of transthyretin Anvesh K. R. Dasar
